胚珠
花粉管
配子体
生物
花粉
卵细胞
植物
不育
人类受精
管(容器)
雄蕊
双受精
绿色荧光蛋白
胚胎
授粉
细胞生物学
基因
解剖
遗传学
工程类
机械工程
作者
Mihaela L. Márton,Simone Cordts,Jean Broadhvest,Thomas Dresselhaus
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2005-01-28
卷期号:307 (5709): 573-576
被引量:276
标识
DOI:10.1126/science.1104954
摘要
Pollen tube guidance precedes the double fertilization of flowering plants. Here, we report the identification of a small maize protein of 94 amino acids involved in short-range signaling required for pollen tube attraction by the female gametophyte. ZmEA1 is exclusively expressed in the egg apparatus, consisting of the egg cell and two synergids. Chimeric ZmEA1 fused to green fluorescent protein (ZmEA1:GFP) was first visible within the filiform apparatus and later was localized to nucellar cell walls below the micropylar opening of the ovule. Transgenic down-regulation of the ZmEA1 gene led to ovule sterility caused by loss of close-range pollen tube guidance to the micropyle.
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