生物
活性氧
黄曲霉
DNA断裂
细胞凋亡
程序性细胞死亡
微生物学
细胞生物学
真菌
生物化学
植物
作者
Xiaoyun Wang,Youzhi Wang,Yu‐Guang Zhou,Xinli Wei
出处
期刊:Mycologia
[Informa]
日期:2014-09-01
卷期号:106 (5): 881-888
被引量:39
摘要
AbstractFarnesol (FOH) is known to induce apoptosis in some fungi and mammalian cells. We treated Aspergillus flavus, one of the leading causes of human invasive aspergillosis and a key producer of the most potent naturally occurring hepatocarcinogenic compounds, with FOH to assess its effect on the viability of the fungus. FOH strongly inhibited germination and growth of A. flavus and induced markers for apoptosis including nuclear condensation, phosphatidylserine (PS) externalization, DNA fragmentation and intracellular reactive oxygen species (ROS) generation, metacaspase activation and abnormal cellular ultrastructure. Moreover, FOH-induced apoptosis in A. flavus was inhibited by the broad-spectrum caspase inhibitor Z-VAD-fmk and partially inhibited by the ROS scavenger l-proline, which suggests that FOH induces apoptosis in A. flavus via a mechanism involving metacaspase activation and ROS production.KeywordsantifungalCa2+metacaspasereactive oxygen species (ROS) AcknowledgmentsThis research was supported by State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences.
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