大肠杆菌
拉伤
新陈代谢
突变体
化学
效价
产量(工程)
甘油
生物
生物化学
基因
遗传学
抗体
解剖
冶金
材料科学
作者
Yingxue Liao,Zhijian Ni,Jinyong Wu,Zhongkui Li,Yongshuai Ge,Xiang-Song Chen,Jianming Yao
标识
DOI:10.1080/13102818.2021.1885996
摘要
2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to improve 2'-FL production. The highest 2'-FL titre of 5.04 g/L, almost 1.4 times higher than that in the control strain, was obtained in the arcA mutant strain using shake-flask culture. In addition, the arcA mutant showed a significantly reduced accumulation of acetate. However, the deletion of iclR and the double deletion of arcA/iclR provided no benefit in terms of 2'-FL biosynthesis. Overall, these results suggest that deletion of arcA could efficiently increase the 2'-FL yield in E. coli and could lay a foundation for more efficient and larger-scale production. Moreover, in shake-flask culture under optimal conditions, the 2'-FL titer reached a maximum value of 7.14 g/L.
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