丝素
柞蚕
粘附
自愈水凝胶
丝绸
细胞粘附
化学
高分子化学
家蚕
高分子科学
化学工程
材料科学
生物化学
复合材料
有机化学
工程类
基因
作者
Boxiang Wang,Hangdan Xu,Jia Li,Dehong Cheng,Yanhua Lü,Li Liu
出处
期刊:RSC Advances
[The Royal Society of Chemistry]
日期:2021-01-01
卷期号:11 (45): 28401-28409
被引量:5
摘要
At present, Antheraea pernyi silk fibroin (ASF) based hydrogels have wide potential applications as biomaterials because of their superior cytocompatibility. Herein, ASF is used as a nucleophilic reagent, reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE). The investigation of ASF-AGE structure by 1H NMR and FTIR are revealed that reactive allyl groups were obtained on ASF by nucleophilic substitution. A series of ASF based hydrogels are manufactured by N-isopropylacrylamide (NIPAAm) copolymerization bridged with ASF-AGE. By the silk fibroin self-assembly process, stably physical cross-linked hydrogels are formed without any crosslinking agent. These hydrogels exhibit good thermoresponsive and degradability, for which the LCST was about 32 °C, and these hydrogels can be degraded in protease XIV solution. Excellent cell proliferation, viability and morphology is demonstrated for b End.3 cells on the hydrogels by the characteristic MTT assay, CLSM and SEM. The cytocompatibility of b End.3 cells was demonstrated with excellent cell adhesion and growth on these ASF based hydrogels in vitro. These degradable and thermoresponsive ASF based hydrogels may find potential applications for cells delivery devices and tissue engineering.
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