Visual Detection of Murray Valley Encephalitis Virus by Reverse Transcription Loop-Mediated Isothermal Amplification

A sensitive reverse transcription loop-mediated isothermal amplification(RT-LAMP)assay was developed for rapid visual detection of Murray valley encephalitis virus(MVEV)infection.The reaction was performed in one step in a single tube at 63°C for 60 min with the addition of the hydroxynaphthol blue(HNB)dye prior to amplification.The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold