Consideration of metabolomics and transcriptomics data in the context of using avian embryos for toxicity testing

代谢组 转录组 生物 代谢组学 背景(考古学) 组学 毒性 计算生物学 生物信息学 遗传学 基因 内科学 基因表达 医学 古生物学
作者
Émilie Legrand,Yeon‐Seon Jeon,Niladri Basu,Markus Hecker,Doug Crump,Jianguo Xia,B. Chandramouli,Heather Butler,Jessica Head
出处
期刊:Comparative Biochemistry and Physiology C-toxicology & Pharmacology [Elsevier BV]
卷期号:258: 109370-109370 被引量:5
标识
DOI:10.1016/j.cbpc.2022.109370
摘要

Early-life stage (ELS) avian toxicity tests have been proposed as a more ethical alternative to traditional standardized tests with adult birds. At the same time, 'omics approaches are gaining traction in the field of avian toxicology, but little has been done to characterize the metabolome and transcriptome at different life stages. The present study uses 'omics data from toxicity tests of 8 environmental chemicals in ELS and adult Japanese quail (Coturnix japonica) to address this data gap. Previous analyses of these data focused on responses to each of the individual chemicals. Here, we consider data from all studies to describe variation in the metabolome and transcriptome between life stages and across independent experiments, irrespective of chemical treatment. Of the 230 metabolites detected in liver, 163 were shared between the two life stages. However, many of the targeted bile acids that were present in the adult liver were absent from ELS samples. For the transcriptome, >90% of the 18,364 detected transcripts were common to both life stages. Based on the 213 genes solely detected in ELS liver, the neuroactive ligand-receptor interaction pathway was significantly enriched. Multivariate and hierarchical clustering analyses revealed that variability among independent experiments was higher for the adult than the ELS studies at both the metabolomic and transcriptomic levels. Our results indicate concordance of the two approaches, with less variation between independent experiments in the ELS metabolome and transcriptome than in adults, lending support for the use of ELS as an alternative toxicity testing strategy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
5秒前
h w wang完成签到,获得积分10
9秒前
Dream完成签到,获得积分0
10秒前
Anna完成签到 ,获得积分10
10秒前
10秒前
点点完成签到 ,获得积分10
11秒前
Hunter完成签到,获得积分10
11秒前
lydiaabc完成签到,获得积分10
16秒前
20秒前
求知小生完成签到,获得积分10
21秒前
26秒前
30秒前
梨里完成签到,获得积分10
35秒前
冯不言发布了新的文献求助10
36秒前
36秒前
啊福完成签到,获得积分10
38秒前
徐彬荣完成签到,获得积分10
38秒前
青檬完成签到 ,获得积分10
40秒前
41秒前
41秒前
量子星尘发布了新的文献求助10
42秒前
44秒前
哈哈哈完成签到,获得积分10
47秒前
47秒前
Balance Man完成签到 ,获得积分0
48秒前
mix完成签到 ,获得积分10
55秒前
小白完成签到,获得积分10
56秒前
瘦瘦的迎梦完成签到 ,获得积分10
57秒前
wwrjj完成签到,获得积分10
57秒前
赵西里完成签到,获得积分10
1分钟前
11完成签到 ,获得积分10
1分钟前
小公牛完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
西洲完成签到 ,获得积分10
1分钟前
1分钟前
xiaofeiyan完成签到 ,获得积分10
1分钟前
笑点低蜜蜂完成签到 ,获得积分10
1分钟前
研学弟完成签到,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Social Research Methods (4th Edition) by Maggie Walter (2019) 2390
A new approach to the extrapolation of accelerated life test data 1000
北师大毕业论文 基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 390
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4008763
求助须知:如何正确求助?哪些是违规求助? 3548409
关于积分的说明 11298823
捐赠科研通 3283064
什么是DOI,文献DOI怎么找? 1810290
邀请新用户注册赠送积分活动 886000
科研通“疑难数据库(出版商)”最低求助积分说明 811220