Phenotyping of CAR T cells

Adaptive immunotherapy has been growing as a therapeutic alternative for cancer and other diseases treatment; chimeric antigen receptor (CAR) T cells comprise a type of adaptive immunotherapy, with recombinant receptor constructs expressed in T cells to target cells expressing specific antigens, is a potential new treatment for blood cancers and solid tumors. The phenotypic, activation, and functional profile of infused CAR-T cells have been linked to the efficacy of CAR-T cell treatment. As a result, immunophenotypic characterization of CAR-T cells during the bioprocess is critical for controlling cell quality and, eventually, improving antitumor effectiveness. Therefore, we propose a flow cytometry based immunophenotyping analysis of CAR-T cells in this chapter to define the phenotype of CAR-T subsets.