生物标志物
医学
药效学
计算生物学
药物开发
生物标志物发现
临床实习
作者
Dianna Y. Wu,Lisa Patti-Diaz,Carla G Hill
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2010-08-20
卷期号:2 (9): 1617-1626
被引量:27
摘要
Flow cytometry is a powerful analytical tool for the analysis of multiple biological parameters of individual cells or particles within heterogeneous cell populations. It has been widely used in biomedical research to perform immunophenotyping, cell counting and numerous cell function assessments, such as intracellular cytokine production, protein phosphorylation, cell proliferation and apoptosis. The implementation of standardized flow cytometry-based biomarker assays in clinical trials remains a challenge due to the limited stability of clinical specimens and the technical variations between instruments. To ensure data quality, it is crucial to develop robust assays for clinical applications. In this review, we summarize current practice in developing, validating and implementing flow cytometry assays to evaluate biomarkers in clinical research.
科研通智能强力驱动
Strongly Powered by AbleSci AI