基因分型
底漆延伸
SNP基因分型
分子反转探针
SNP公司
生物
基因座(遗传学)
等位基因
遗传学
底漆(化妆品)
基因型
寡核苷酸
单核苷酸多态性
分子生物学
化学
基序列
DNA
基因
有机化学
作者
Stacey Gabriel,Liuda Ziaugra,Diana Tabbaa
标识
DOI:10.1002/0471142905.hg0212s60
摘要
The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.
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