弥黑
脂肪酶
化学
咪唑酯
水溶液
锌
生物催化
摩尔比
核化学
荧光假单胞菌
沸石咪唑盐骨架
催化作用
金属有机骨架
有机化学
甘油三酯酶
酶
反应机理
吸附
细菌
生物
遗传学
作者
Federica Pitzalis,Cristina Carucci,Maryam Naseri,Lida Fotouhi,Edmond Magner,Andrea Salis
出处
期刊:Chemcatchem
[Wiley]
日期:2018-03-07
卷期号:10 (7): 1578-1585
被引量:43
标识
DOI:10.1002/cctc.201701984
摘要
Abstract Lipase AK from Pseudomonas fluorescens and Lipase RM from Rhizomucor miehei were encapsulated into a zeolite imidazolate framework (ZIF‐8) by a “one‐pot” synthesis to obtain AK@ZIF‐8 and RM@ZIF‐8 biocatalysts. The effect of a high (1:40) and low (1:4) Zn/2‐methylimidazole molar ratio on the biocatalysts synthesis was investigated. The different Zn/ligand (L) ratios affected both the surface area, the loading, and the specific activity of the biocatalysts. Samples synthesized by using a high Zn/L ratio had high values of surface area whereas those obtained by using a low Zn/L ratio had higher loadings and specific activities. The decrease of pH (from 11.6 to 9.4) during the synthesis at high Zn/L ratio produced ZIF‐8 samples with features similar to those observed for low Zn/L ratio samples. The low Zn/L (1:4) ratio AK@ZIF‐8 biocatalyst retained 99 % activity after storage for 15 days at 5 °C and 40 % activity after five reaction cycles.
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