The contents of hesperidin were determined in citrus peel,citrus flesh,dried tangerine fibre and citrus seed by UPLC.The results showed hesperidin could be separated with other substances in the provided chromatography conditions.The contents of hesperidin in citrus peel,citrus flesh,dried tangerine fibre and citrus seed were 332.21,50.85,581.84 and 641.73 mg / kg,respectively.The method can be used to determine the content of hesperidin in citrus and related products.