Effect of radiation on chemical composition and protein quality of cottonseed meal

Abstract This study was conducted to compare the effects of electron beam ( EB ) and gamma ray (GR) irradiation treatments at doses of 10, 20 and 30 kG y on chemical composition, protein quality and protein digestibility of cottonseed meal ( CSM ). GR irradiation in all doses significantly decreased the crude fiber of samples compared to raw CSM . Free and total gossypol content of CSM was decreased significantly by utilizing both types of irradiation in a dose‐dependent manner. Also, EB irradiation caused decrease in free and total gossypol content more than that of GR irradiation. GR irradiation at doses of 20 and 30 kG y, and EB irradiation at doses of 10, 20 and 30 kG y can significantly decrease protein solubility of CSM compared to that of a raw sample. GR irradiation at a dose of 30 kG y significantly increased apparent digestibility of protein compared to raw and EB irradiation of CSM at a dose of 10 kG y in Leghorn cockerels. Maximum increase in protein digestibility of CSM was observed in GR irradiation at a dose of 30 kG y. In conclusion, the present study showed that EB and GR irradiation reduced gossypol and crude fiber and increased protein digestibility of CSM but had no effect on protein quality of CSM.