胚泡
生物
男科
胚胎
细胞生物学
DNA断裂
转录组
合子
胚胎发生
基因表达
基因
细胞凋亡
遗传学
程序性细胞死亡
医学
作者
Alisa Komsky-Elbaz,Dorit Kalo,Zvi Roth
出处
期刊:Reproduction
[Bioscientifica]
日期:2020-11-01
卷期号:160 (5): 709-723
被引量:13
摘要
This study aims to evaluate the deleterious effect of the mycotoxin aflatoxin B1 (AFB1) on bull spermatozoa and the carryver effect on the developing embryo. Proteomic analysis of AFB1-treated spermatozoa revealed differential expression of proteins associated with biological processes and cellular pathways that involved in spermatozoon function, fertilization competence and embryonic development. Therefore, we assume that factors delivered by the spermatozoa, regardless of DNA fragmentation, are also involved. To confirm this hypothesis, we have used the annexin V (AV) kit to separate the spermatozoa into apoptotic (AV+) and non-apoptotic (AV−) subpopulations which were found to correlate with high- and low DNA fragmentation, respectively. Fertilization with AV+ AFB1-treated spermatozoa, resulted in no blastocyst formation, whereas fertilization with AV− spermatozoa resulted in reduced cleavage rate and formation of genetically altered blastocysts ( POU5F1 and SOX2 ). Microarray analysis of blastocysts derived from 10 µM AFB1-treated spermatozoa revealed differential expression of 345 genes that involved in cellular pathways such as embryo and placenta development, cell cycle, DNA repair and histone modification, and in signaling pathways, especially calcium signaling pathway. This is the first report on deleterious carrying over effects of AFB1 from the bovine spermatozoa to the formed embryo. Our findings suggest that aside from the damage caused by AFB1 to spermatozoa’s DNA integrity, additional damage mechanisms are involved.
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