PI3K/AKT/mTOR通路
细胞凋亡
蛋白激酶B
细胞周期
细胞生物学
生殖毒性
流式细胞术
化学
生物
毒性
信号转导
分子生物学
生物化学
有机化学
作者
Yongya Wu,Jun Ma,Yufei Sun,Meng Tang,Lu Kong
出处
期刊:Chemosphere
[Elsevier]
日期:2020-09-01
卷期号:255: 126913-126913
被引量:39
标识
DOI:10.1016/j.chemosphere.2020.126913
摘要
Nickel nanoparticles (Ni NPs) have a wide range of application prospects, but there is still a lack of their safety evaluation for the reproductive system. Nowadays, male reproductive health has been widely concerned because of the increasing incidence of male infertility. Studies have shown that Ni NPs can cause male reproductive toxicity. The purpose of this study was to investigate the toxicity of Ni NPs on GC-1 cells, a mouse spermatogonia cell line, and to explore the possible mechanism underlying the induction of apoptosis via PI3K/AKT/mTOR signaling pathway. The cell ultrastructure was firstly observed under a transmission electron microscope. Then, cell proliferation, cycle and apoptosis were detected by CCK-8 and flow cytometry, respectively. Furthermore, the expression levels of related proteins and genes were determined by Western blot and Reverse transcription-polymerase chain reaction, respectively. The results showed that Ni NPs could not only cause changes in cell ultrastructure, decreased survival rate and arrested G1 phase cell cycle, but also activated apoptosis pathway by inhibiting the PI3K/AKT/mTOR signaling pathway. The results of this study provide novel insights to explore the mechanisms of reproductive toxicity of Ni NPs and are of great significance to develop safety evaluation criteria for Ni NPs.
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