白色念珠菌
几丁质酶
甲壳素
酵母
离心
动力学
化学
酶
几丁质合成酶
基质(水族馆)
生物化学
酶分析
水解
色谱法
生物
壳聚糖
微生物学
物理
量子力学
生态学
作者
Keith Barrett‐Bee,Mark Hamilton
出处
期刊:Microbiology
[Microbiology Society]
日期:1984-07-01
卷期号:130 (7): 1857-1861
被引量:12
标识
DOI:10.1099/00221287-130-7-1857
摘要
Chitinase activity was detected in the supernatant fraction of a high-speed centrifugation preparation of broken Candida albicans yeast cells. The enzyme showed peak activity during the rapid budding phase of growth and was found to parallel the chitin synthase activity. The optimum conditions for the hydrolysis of chitin, regenerated from acetylation of chitosan, were determined. Analysis of the kinetics of the enzyme-substrate interaction and a measurement of their binding suggests that an equilibrium binding situation exists and that the kinetics follow a Langmuir isotherm interaction.
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