光电流
化学
纳米技术
异质结
小RNA
微泡
载流子
生物传感器
光电子学
材料科学
生物化学
基因
作者
Y. Wang,Mengchun Yang,Shenguang Ge,Xiao Wang,Jinghua Yu
标识
DOI:10.1021/acs.analchem.2c02821
摘要
Exosomal microRNAs (miRNAs) as newly emerging reliable and noninvasive biomarkers have demonstrated a significant function in early cancer diagnosis. Photoelectrochemical (PEC) biosensing has attracted unprecedented attention in exosomal miRNA monitoring due to its inherent advantages of both electrochemical and optical techniques; however, the severe charge carrier recombination greatly restricts the PEC assay performance. Herein, a high-sensitive PEC strategy assisted by the piezoelectric effect is designed based on Bi2WO6/Cu2S heterojunctions and implemented for the monitoring of exosomal miRNAs. The introduction of the piezoelectric effect enables promoted electron-hole transfer and separation, thereby improving the analytical sensitivity. In addition, a target reprogramming metal-organic framework-capped CaO2 (MOF@CaO2) hybrids is prepared, in which MOF@CaO2 being responsive to exosomal miRNAs induces exposure of the capped CaO2 to H2O and then triggers self-supplying of H2O2, which effectively suppresses the electron-hole recombination, giving rise to an amplified photocurrent and a decrease in the cost of the reaction. Benefiting from the coupled sensitization strategy, the as-fabricated PEC strategy exhibits high sensitivity, specificity, low cost, and ease of use for real-time analysis of exosomal miRNAs within the effectiveness linear range of 0.1 fM-1 μM. The present work demonstrates promising external field coupling-enhanced PEC bioassay and offers innovative thoughts for applying this strategy in other fields.
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