Mixed biofilm formation by Oenococcus oeni and Saccharomyces cerevisiae: A new strategy for the wine fermentation process

Bacterial biofilms have attracted much attention in the food industry since this phenotype increases microbial resistance to environmental stresses. In wine-making, the biofilm produced by Oenococcus oeni is able to persist in this harsh environment and perform malolactic fermentations. Certain viticultural practices are interested in the simultaneous triggering of alcoholic fermentation by yeasts of the species Saccharomyces cerevisiae and malolactic fermentation by lactic acid bacteria. As yet, no data is available on the ability of these micro-organisms to produce mixed biofilms and promote fermentations. Here, the ability of S. cerevisiae and O. oeni to form mixed biofilms on different surfaces found in vinification was observed and analyzed using scanning electron microscopy experiments. Then, following co-inoculation with biofilm or planktonic cells microvinifications were carried out to demonstrate that the mixed biofilms developed on oak allow the efficient completion of fermentations because of their high resistance to stress. Finally, comparisons of the different metabolic profiles obtained by LC-MS were made to assess the impact of the mode of life of biofilms on wine composition.