Electrochemiluminescent biosensor based on AuNPs@PEI/MWCNTs and OMC@Ru(bpy)32+ for the detection of Vibrio parahaemolyticus

Vibrio parahaemolyticus (VP) is one of the most common pathogens in aquatic products, and its study using electrochemiluminescence is still relatively rare. In this study, an electrochemiluminescent (ECL) biosensor was developed to detect VP in aquatic products using AuNPs modified PEI/MWCNTs (AuNPs@PEI/MWCNTs) as a substrate material and ordered mesoporous carbon (OMC) adsorbed Ru(bpy)32+ (OMC@Ru(bpy)32+) as a luminescent material. To increase the conductivity of the biosensor and offer a favorable loading environment for the immobilization of the luminescent probe, AuNPs@PEI/MWCNTs are employed as the substrate. Meanwhile, the OMC@Ru(bpy)32+ functionalized DNA probe acts as a luminescent and capture probe to generate ECL signals and specifically recognize different concentrations of VP. The biosensor has good selectivity and stability and has been successfully applied to the detection of VP in scallops. This method not only expands the potential uses of bioprobe-based ECL biosensors, but also offers a new and effective approach used to detect food-borne pathogens in aquatic products.