黄曲霉毒素
化学
吸附
黄曲霉
色谱法
核化学
解吸
粒径
分子印迹
磁性纳米粒子
纳米颗粒
选择性
有机化学
纳米技术
材料科学
食品科学
催化作用
物理化学
作者
Veli Ziya Erdem,Hatice İmge Oktay Başeğmez,Gözde Baydemir Peşint
标识
DOI:10.1016/j.jchromb.2022.123453
摘要
Aflatoxins (AFs) are produced mainly by Aspergillus flavus and Aspergillus parasiticus and aflatoxin B1 (AFB1) is one of the most toxic aflatoxins with its carcinogenic property. AFB1 recognition from samples is very important and PHEMA based AFB1 imprinted magnetic nanoparticles (magAFB1-MIPs) were synthesized for the selective AFB1 recognition from liver tissue. The AFB1-MIPs were synthesized in different mole ratios and NIPs were synthesized for control. Characterization studies of magAFB1-MIPs and NIPs were carried out by swelling tests, surface area measurements, scanning electron microscopy and particle size analysis. The surface area was found as 117 m2/g and the size of the nanoparticles were found as 483 nm in diameter. The percentage yield of polymerization was calculated as 98 % and the template (AFB1) removal ratio from the magAFB1-MIPs was calculated as 91 %. The maximum adsorbtion capacities were calculated as 427.57 ng g−1 for magAFB1-MIPs and 44.6 ng g−1 for magNIPs. Selectivity tests showed that magAFB1-MIPs adsorb AFB1 1.74, 4.40, 2.46 times selective than that of AFB2, AFG1 and AFG2 molecules, respectively. AFB1 removal amount from AFB1 spiked liver tissue was satisfactory and recorded as 10.4 ng g−1 and 54.8 ng g−1 for 2 ng g−1 and 10 ng g−1 spiked liver tissue samples, respectively. AFB1 adsorption amount decrease was found negligible for 10 consecutive adsorption–desorption repeats in reusability study.
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