免疫分析
生物技术
转基因生物
定量蛋白质组学
可靠性
质谱法
生化工程
作物
计算生物学
蛋白质组学
生物
化学
色谱法
农学
工程类
生物化学
基因
政治学
抗体
法学
免疫学
作者
Y. H. Wang,Mark Bednarcik,Christopher Ament,Matthew L. Cheever,Simone M. Cummings,Tao Geng,Dulan B. Gunasekara,Norma L. Houston,Kristen Kouba,Lu Zi,Jeffrey Shippar
标识
DOI:10.1021/acs.jafc.3c09188
摘要
Quantifying protein levels in genetically modified (GM) crops is crucial in every phase of development, deregulation, and seed production. Immunoassays, particularly enzyme-linked immunosorbent assay, have been the primary protein quantitation techniques for decades within the industry due to their efficiency, adaptability, and credibility. Newer immunoassay technologies like Meso Scale Discovery and Luminex offer enhanced sensitivity and multiplexing capabilities. While mass spectrometry (MS) has been widely used for small molecules and protein detection in the pharmaceutical and agricultural industries (e.g., biomarkers, endogenous allergens), its use in quantifying protein levels in GM crops has been limited. However, as trait portfolios for GM crop have expanded, MS has been increasingly adopted due to its comparable sensitivity, increased specificity, and multiplexing capabilities. This review contrasts the benefits and limitations of immunoassays and MS technologies for protein measurement in GM crops, considering factors such as cost, convenience, and specific analytical needs. Ultimately, both techniques are suitable for assessing protein concentrations in GM crops, with MS offering complementary capabilities to immunoassays. This comparison aims to provide insights into selecting between these techniques based on the user's end point needs.
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