绿色荧光蛋白
骨肉瘤
转染
转移
癌症研究
医学
肺
病理
原发性肿瘤
裸鼠
癌症
细胞培养
生物
内科学
基因
生物化学
遗传学
作者
Hiromichi Oshiro,Yasunori Tome,Kentaro Miyake,Takashi Higuchi,Norihiko Sugisawa,Jun Ho Park,Zhiying Zhang,Sahar Razmjooei,Fuminori Kanaya,Kotaro Nishida,Robert M. Hoffman
出处
期刊:Anticancer Research
[Anticancer Research USA Inc.]
日期:2021-01-30
卷期号:41 (2): 635-640
被引量:1
标识
DOI:10.21873/anticanres.14814
摘要
Background: A mouse model of metastatic osteosarcoma is imperative to identify effective agents for metastatic osteosarcoma, which is a recalcitrant disease. In the present study, we established osteosarcoma patient-derived cells (OS-PDCs) and transfected them with green fluorescent protein (GFP). Materials and Methods: The OS-PDCs were transfected with GFP-lentivirus. GFP-expressing OS-PDCs (2.0×105) were then injected into the tibia of nude mice to establish the patient-derived orthotopic cell (PDOC) model (n=3). Six weeks after injection, the primary tumor and each organ were resected and imaged. Results: Primary orthotopic tumors were established in two out of three mice. The GFP-expressing OS-PDCs in the PDOC model were visualized. Multiple GFP-expressing lung metastases were detected in one of the two mice with primary tumor. Conclusion: The present study proves the concept that a GFP-expressing PDOC model can mimic clinical lung-metastatic osteosarcoma. This model can serve as a paradigm to screen for effective drugs for osteosarcoma lung metastasis.
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