聚酮
生物合成
非核糖体肽
聚酮合酶
异源表达
异源的
大肠杆菌
合成生物学
代谢工程
生物
生物化学
重组DNA
计算生物学
基因
作者
Xiangyang Li,Kangmin Hua,Dongxu Li,Zhen‐Long Wu,Ying Wang,Haoran Zhang,Zixin Deng,Blaine A. Pfeifer,Ming Jiang
标识
DOI:10.1021/acschembio.9b00827
摘要
The heterologous biosynthesis of complex natural products has enabled access to polyketide, nonribosomal peptide, isoprenoid, and other compounds with wide-spanning societal value. Though several surrogate host systems exist, Escherichia coli is often a preferred choice due to its rapid growth kinetics and extensive molecular biology protocols. However, a persistent challenge to the utilization of E. coli has been the successful in vivo reconstitution of type II polyketide synthase (PKS) systems. In particular, gene expression of the ketosynthase (KS) components of the minimal PKS has consistently yielded insoluble protein products. In the following report, two type II PKS systems were functionally reconstituted in E. coli. The approach to do so relied upon the utilization of the native transcriptional coupling between the dimeric KS subunits, leading to soluble recombinant protein products and successful polyketide biosynthesis. Resulting strains produced 10 mg/L TW95c and 25 mg/L dehydrorabelomycin. Hence, the strategy offers a new option in the biosynthetic engineering efforts for the heterologous production of type II polyketide products using E. coli.
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