Ultrasensitive detection of bisphenol A under diverse environments with an electrochemical aptasensor based on multicomponent AgMo heteronanostructure

A novel series of bimetallic AgMo heteronanostructures (Ag-PMo12) were prepared by annealing silver ions doped phosphomolybdic acid (H3PMo12O40) at different temperatures (300, 600, and 800 °C), and explored as the sensitive platforms for developing electrochemical biosensors to detect the trace bisphenol A (BPA). Amongst, Ag-PMo12 calcinated at 600 °C gave a multicomponent nanohybrid of Ag, Ag2O, Ag2S, and ultra-thin MoS2 nanosheet (denoted as Ag/Ag2O/Ag2S/MoS2(600)). This nanohybrid exhibited active electrochemical performance, excellent biocompatibility, strong bioaffinity and superior adsorbability to aptamer strands, leading to optimal sensing performance in comparison with other hybrids. As a result, the Ag/Ag2O/Ag2S/MoS2(600)-based aptasensor displayed an ultralow detection limit of 0.2 fg mL−1 within the BPA concentration of 1–1000 fg mL−1. This aptasensor also showed high selectivity and good stability, reproducibility, regenerability, and applicability in different situations. This result will not only develop an excellent sensing platform toward BPA detection in aspects of environmental monitoring, food safety, and human health, but also present a promising sensing strategy that can be extensively explored to detect other targets via anchoring of various aptamer strands.