转录组
生物
核糖核酸
细胞
髓样
人口
移植
电池类型
计算生物学
RNA序列
细胞生物学
基因
肾
免疫学
遗传学
基因表达
医学
内科学
环境卫生
作者
Elly Varma,Xunrong Luo,Thangamani Muthukumar
出处
期刊:Current Opinion in Organ Transplantation
[Ovid Technologies (Wolters Kluwer)]
日期:2020-12-10
卷期号:26 (1): 43-51
被引量:11
标识
DOI:10.1097/mot.0000000000000840
摘要
Purpose of review Single-cell RNA sequencing (scRNA-seq) has provided opportunities to interrogate kidney allografts at a hitherto unavailable molecular level of resolution. Understanding of this technology is essential to better appreciate the relevant biomedical literature. Recent findings Sequencing is a technique to determine the order of nucleotides in a segment of RNA or DNA. RNA-seq of kidney allograft tissues has revealed novel mechanistic insights but does not provide information on individual cell types and cell states. scRNA-seq enables to study the transcriptome of individual cells and assess the transcriptional differences and similarities within a population of cells. Initial studies on rejecting kidney allograft tissues in humans have identified the transcriptional profile of the active players of the innate and adaptive immune system. Application of scRNA-seq in a preclinical model of kidney transplantation has revealed that allograft-infiltrating myeloid cells follow a trajectory of differentiation from monocytes to proinflammatory macrophages and exhibit distinct interactions with kidney allograft parenchymal cells; myeloid cell expression of Axl played a major role in promoting intragraft myeloid cell and T-cell differentiation. Summary The current review discusses the technical aspects of scRNA-seq and summarizes the application of this technology to dissect the human kidney allograft transcriptome.
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