生物
16S核糖体RNA
副干酪乳杆菌
植物乳杆菌
乳酸菌
鼠李糖乳杆菌
微生物学
核糖体RNA
细菌
食品科学
发酵
基因
乳酸
遗传学
作者
Ait Abdeslam Arezki,Nathalie Desmasures,Bakr Ahmed
出处
期刊:South Asian Journal of Experimental Biology
日期:2020-01-01
卷期号:9 (5): 193-206
被引量:2
标识
DOI:10.38150/sajeb.9(5).p193-206
摘要
El-klila, Jben, Rayeb and Lben are artisanal dairy products typical of the North African region. These products are considered a good source of nutrients and a vector par excellence of indigenous lactic acid bacteria with significant technological and therapeutic potential. In this study, fifty-two Lactobacillus isolates from traditional dairy products (El-Klila, Jben and Rayeb) from the Ain Sefra region (southwestern of Algeria) were identified by phenotypic, genotypic and MALDI-TOF mass spectrometry methods. Phenotypic characterization allowed dividing the isolates into two groups: 7.69% of isolates mesophilic and strict heterofermentative and 92.31% isolates mesophilic and optional heterofermentative. The isolates identification by sequencing 16S rDNA was performed using the universal primers w02 and w18. Comparing the sequences of the isolates with the GenBank database (NCBI) revealed a high percentage with five species of Lactobacillus. Of the 52 isolates, thirteen were identified as Lactobacillus paracasei, four isolates were L. brevis, five isolates were L. rhamnosus and ten isolates were L. plantarum. The lasting twenty isolates were related, at the same time, to the plantarum species and the pentosus species. The use of MALDI-TOF mass spectrometry confirmed the identity of 36 strains of Lactobacillus. Of the 20 isolates related to the plantarum / pentosus group by 16S rDNA, 50% were identified as L. plantarum. Finally, the results of the two combined methods allowed us to correctly identify 80.76% of the Lactobacillus isolates at the genus and species level. The analysis by MALDI-TOF SM has proved to be a very reliable, fast (less than an hour) and inexpensive tool for identifying native Lactobacillus species from artisanal dairy products. This method can constitute a major tool for the study of the microbial biodiversity of artisanal food products.
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