多路复用
循环肿瘤细胞
微流控
仿形(计算机编程)
生物标志物
计算生物学
表型
纳米技术
材料科学
计算机科学
生物
生物信息学
癌症
基因
遗传学
转移
操作系统
作者
Lingling Wu,Zhiling Zhang,Man Tang,Daoben Zhu,Xianguang Dong,Jiao Hu,Changxing Qi,Hong‐Wu Tang,Dai‐Wen Pang
标识
DOI:10.1002/anie.201914468
摘要
Abstract Comprehensive phenotypic profiling of heterogeneous circulating tumor cells (CTCs) at single‐cell resolution has great importance for cancer management. Herein, a novel spectrally combined encoding (SCE) strategy was proposed for multiplex biomarker profiling of single CTCs using a multifunctional nanosphere‐mediated microfluidic platform. Different cellular biomarkers uniquely labeled by multifunctional nanosphere barcodes, possessing identical magnetic tags and distinct optical signatures, enabled isolation of heterogeneous CTCs with over 91.6 % efficiency and in situ SCE of phenotypes. By further trapping individual CTCs in ordered microstructures on chip, composite single‐cell spectral signatures were conveniently and efficiently obtained, allowing reliable spectral‐readout for multiplex biomarker profiling. This SCE strategy exhibited great potential in multiplex profiling of heterogeneous CTC phenotypes, offering new avenues for cancer study and precise medicine.
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