[Effects of zinc ions on biological functions of human umbilical vein endothelial cells].

Objective: To evaluate the effect of zinc ions on human umbilical vein endothelial cells biological functions. Methods: The primary human umbilical vein endothelial cells were cultured with the ECM medium, and cells were divided into 8 groups: the control group(routine culture,n=3), 20 μmol/L zinc group(20 μmol/L zinc chloride solution was added into the cell medium, n=3), 40 μmol/L zinc group(40 μmol/L zinc chloride solution was added into the cell medium, n=3),80 μmol/L zinc group(80 μmol/L zinc chloride solution was added into the cell medium, n=3), 100 μmol/L zinc group(100 μmol/L zinc chloride solution was added into the cell medium, n=3), 200 μmol/L zinc group(200 μmol/L zinc chloride solution was added into the cell medium, n=3),300 μmol/L zinc group(300 μmol/L zinc chloride solution was added into the cell medium, n=3), 500 μmol/L zinc group(500 μmol/L zinc chloride solution was added into the cell medium, n=3). The cell proliferation curve was derived from real time cell analysis (RTCA). The viability value was obtained via CCK-8 reagent, and the migration distance was tested by scratch-wound assay while the adhesion function was detected by RTCA. Results: (1)After 18 hours, RTCA showed that the proliferation cell indexes were 4.5±0.6, 3.7±0.4, 3.6±0.3, 2.5±0.4, and 2.5±0.4 in the 20, 40, 80, 100, and 200 μmol/L zinc groups, as compared with 3.5±0.3 in the control group (all P<0.05). Proliferation cell indexes were 0 in both of the 300 μmol/L and 500 μmol/L zinc groups. (2)After 96 hours, the viability were 1.21±0.05, 1.10±0.03, 0.99±0.05, 0.62±0.02, 0.45±0.04, 0.11±0.01, and 0.12±0.06, respectively in the 20, 40, 80, 100, 200, 300, and 500 μmol/L zinc groups, as compared with 0.75±0.05 in the control group (all P<0.05). (3)After 12 hours, the migration distances were (0.56±0.11),(0.96±0.07),(0.49±0.02), and (0.29±0.01)mm in the 20, 40, 80, and 100 μmol/L zinc groups, as compared with (0.24±0.04)mm in the control group (all P<0.05). (4)After 18 hours, the adhesion cell index were 0.40±0.05, 0.31±0.01, 0.38±0.05, and 0.40±0.03 in the 20, 40, 80, and 100 μmol/L zinc groups, as compared with 0.24±0.04 in the control group (all P>0.05). Conclusions: Zinc ions at lower concentration (≤80 μmol/L) can promote proliferation, viability and migration of human umbilical vein endothelial cells, but the adhesion function was not significantly affected by zinc ions. Zinc ions at higher concentration (≥200 μmol/L) can inhibit the cellular function of the human umbilical vein endothelial cells.目的： 探讨锌离子对人脐静脉内皮细胞生物学功能的影响。 方法： 体外原代培养人脐静脉内皮细胞后，传代培养并将细胞分为8组：对照组（常规培养，n=3）；20 μmol/L组（细胞培养液中加入氯化锌溶液20 μmol/L，n=3）；40 μmol/L组（细胞培养液中加入氯化锌溶液40 μmol/L，n=3）；80 μmol/L组（细胞培养液中加入氯化锌溶液80 μmol/L，n=3）；100 μmol/L组（细胞培养液中加入氯化锌溶液100 μmol/L，n=3）；200 μmol/L组（细胞培养液中加入氯化锌溶液200 μmol/L，n=3）；300 μmol/L组（细胞培养液中加入氯化锌溶液300 μmol/L，n=3）；500 μmol/L组（细胞培养液中加入氯化锌溶液500 μmol/L，n=3）。采用实时无标记细胞分析仪检测内皮细胞增殖情况，采用CCK-8法检测内皮细胞活性，采用细胞划痕实验检测内皮细胞迁移能力，同时采用实时无标记细胞分析仪检测细胞黏附功能的变化。 结果： （1）18 h后，20、40、80、100和200 μmol/L组的细胞增殖指数分别为4.5±0.6、3.7±0.4、3.6±0.3、2.5±0.4和2.5±0.4，与对照组的3.5±0.3比较，差异均有统计学意义（P均<0.05）；300和500 μmol/L组的细胞增殖指数均为0。（2）96 h后，20、40、80、100、200、300和500 μmol/L组的细胞活性分别为1.21±0.05、1.10±0.03、0.99±0.05、0.62±0.02、0.45±0.04、0.11±0.01和0.12±0.06，与对照组的0.75±0.05比较，差异均有统计学意义（P均<0.05）。（3）12 h后，20、40、80和100 μmol/L组的细胞迁移距离分别为（0.56±0.11）、（0.96±0.07）、（0.49±0.02）和（0.29±0.01）mm，与对照组的（0.24±0.04）mm比较，差异均有统计学意义（P均<0.05）。（4）18 h后，20、40、80和100 μmol/L组的细胞黏附指数分别为0.40±0.05、0.31±0.01、0.38±0.05和0.40±0.03，与对照组的0.35±0.02比较，差异均无统计学意义（P均>0.05）。 结论： 低浓度锌离子（≤80 μmol/L）对人脐静脉内皮细胞的增殖、活性和迁移功能有促进作用，对黏附功能无明显影响，而较高浓度锌离子（≥200 μmol/L）对细胞功能有抑制作用。.