转基因
生物
重组酶
成纤维细胞
细胞生物学
分子生物学
表型
同源重组
位点特异性重组
原肠化
间充质干细胞
基因
遗传学
重组
细胞培养
胚胎干细胞
作者
Victoire Cardot‐Ruffino,Véronique Chauvet,Cassandre Caligaris,Adrien Bertrand‐Chapel,Nicolas Chuvin,Roxane M. Pommier,Ulrich Valcourt,David F. Vincent,Sylvie Martel,Sophie Aires,Bastien Kaniewski,Pierre Dubus,Philippe Cassier,Stéphanie Sentis,Laurent Bartholin
出处
期刊:Genesis
[Wiley]
日期:2020-03-19
卷期号:58 (5)
被引量:3
摘要
Summary Recombination systems represent a major breakthrough in the field of genetic model engineering. The Flp recombinases (Flp, Flpe, and Flpo) bind and cleave DNA Frt sites. We created a transgenic mouse strain ([Fsp1‐Flpo]) expressing the Flpo recombinase in fibroblasts. This strain was obtained by random insertion inside mouse zygotes after pronuclear injection. Flpo expression was placed under the control of the promoter of Fsp1 (fibroblast‐specific protein 1) gene, whose expression starts after gastrulation at Day 8.5 in cells of mesenchymal origin. We verified the correct expression and function of the Flpo enzyme by several ex vivo and in vivo approaches. The [Fsp1‐Flpo] strain represents a genuine tool to further target the recombination of transgenes with Frt sites specifically in cells of mesenchymal origin or with a fibroblastic phenotype.
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