胰腺癌
EPH受体A2
生物标志物
化学发光
细胞外小泡
癌症
医学
癌症研究
内科学
化学
受体
生物
色谱法
生物化学
细胞生物学
受体酪氨酸激酶
作者
Wenhan Li,Xiaojun Yang,Tingting Bai,Junwen Xu,Zhiguo Qian,Yawen Li,Zhirui Guo,Yong Zhu
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:14 (13): 1335-1341
被引量:1
摘要
Pancreatic cancer has led to an extremely high mortality rate because of its insidious onset and lack of early clinical symptoms. Effective early diagnosis is essential to improve the treatment of pancreatic cancer. Tumor-secreted extracellular vesicles (EVs) have attracted great interest as potential tumor biomarkers. However, most of the methods for detecting serum EVs have some general problems such as cumbersome, time-consuming extraction steps, and high cost, which limit greatly the research on cancer detection based on EVs. Herein, we report a light-initiated chemiluminescent assay (LICA) method using photosensitive beads for direct detection of EVs in serum enriched with ephrin type-A receptor 2 (EphA2), which show high expression in pancreatic cancer patients. Combining with a serum biomarker CA19-9, pancreatic cancer patients could be distinguished rapidly by sensitive detection of EphA2-EVs from serum without any purification. This developed method could be extended to improve the diagnosis efficiency for other cancers and gain an insight into EV detection.
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