生物
细菌
微生物学
噬菌体
基因
大肠杆菌
毒力
生物膜
基因组
基因表达
遗传学
作者
Marta Lourenço,Lorenzo Chaffringeon,Quentin Lamy-Besnier,Marie Titécat,Thierry Pédron,Odile Sismeiro,Rachel Legendre,Hugo Varet,Jean-Yves Coppée,Marion Bérard,Luisa De Sordi,Laurent Debarbieux
标识
DOI:10.1016/j.chom.2022.03.014
摘要
Summary
Abundance and diversity of bacteria and their viral predators, bacteriophages (phages), in the digestive tract are associated with human health. Particularly intriguing is the long-term coexistence of these two antagonistic populations. We performed genome-wide RNA sequencing on a human enteroaggregative Escherichia coli isolate to identify genes differentially expressed between in vitro conditions and in murine intestines. We experimentally demonstrated that four of these differentially expressed genes modified the interactions between E. coli and three virulent phages by either increasing or decreasing its susceptibility/resistance pattern and also by interfering with biofilm formation. Therefore, the regulation of bacterial genes expression during the colonization of the digestive tract influences the coexistence of phages and bacteria, highlighting the intricacy of tripartite relationships between phages, bacteria, and the animal host in intestinal homeostasis.
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