基因沉默
功能基因组学
病毒载体
生物
基因组学
载体(分子生物学)
基因
计算生物学
生物技术
计算机科学
遗传学
基因组
重组DNA
作者
Gaurav Kumar,Kamlesh Kumari,Indranil Dasgupta
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 117-131
被引量:1
标识
DOI:10.1007/978-1-0716-1875-2_8
摘要
The availability of protocols for virus-induced gene silencing (VIGS) in rice has opened up an important channel for the elucidation of gene functions in this important crop plant. Here, we present an updated protocol of a VIGS system based on Rice tungro bacilliform virus (RTBV) for gene silencing in rice. We present complete updated protocols for VIGS in rice, compare the system with other existing ones for monocots, identify some of the challenges faced by this system and discuss ways in which the vector could be improved for better silencing efficiency.
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