The present experiment was conducted to investigate the effect of adding S and/or Ni on the concentration of Ni and Mil in the rhizosphere and metal content in plant parts of wheat seedlings by using a rhizobox system.The results indicate slight differences in pH values of rhizoshpere soil compared to that of bulk soil. The increase in solubility of Ni and Mn was most pronounced in the rhizosphere soil compared to that of the bulk soil. The concentrations of Ni and Mn in plant parts were more related to the contents in the rhizosphere soil compared to that of the bulk soil.It was concluded that the distribution of the metals across the rhizosphere is more important for the evaluation of the availability of heavy metals than their solubility in the bulk soil.