A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells

Cysteine (Cys), the only amino acid in the 20 natural amino acids that contains a reduced sulfhydryl group, plays important roles in the balance of redox homeostasis in biological systems. Lysosome is an important organelle containing a variety of hydrolases and has been proved to be the decomposition center of a variety of exogenous and endogenous macromolecular substances. In this research, a coumarin-based fluorescent probe MCA for the detection of Cys in lysosomes of living cells was developed. Due to the acrylate moiety, this probe exhibited high sensitivity (detection limit = 6.8 nM) and selectivity towards Cys superior to other analytes. Moreover, the probe was proved to be lysosome-targetable and showed good cell imaging ability and low cell toxicity.