内输蛋白
转染
核运输
生物素化
结合
核定位序列
基因传递
分子生物学
化学
细胞质
细胞生物学
重组DNA
生物
生物物理学
作者
Takeshi Kawazu,Hiroyuki Kanzaki,Atsushi Uno,Hideki Azuma,Takeshi Nagasaki
标识
DOI:10.1016/j.biopha.2012.02.005
摘要
In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.
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