Self-screening of high-yield β-glucosidase strain-N_1 for Preparation of soy isoflavone aglycone

Objective: To explore a self-screening of high-yield β-glucosidasestrain-N1forpreparation of soy isoflavone aglycone.Method:Application of conventional methods of enzymatic studies in the lab conditions,using the self-screening of high-yield β-glucosidase strain-N1to obtain β-glucosidase solution,to explore the preparation of soy iso isoflavone aglycone.Result:Lab prepared samples of soy isoflavones aglycone 91.33%purity and the yield was 11.44%.Crude soy isoflavone treated to reduce the number of glycosides,Daidzin and glycitin down to 28.21 percent from 22.93 percent,genistin reduced to 8.24 percent from 5.67 percent.Aglycone significantly increase the number,Daidzein increased to 0.04 percent from 0.05 percent,glycitein increased to 0.003 percent from 0.09 percent,genistein increased to 0.006 percent from 0.07 percent.Conclusion:Research shows that self-screening strain-N1 as the preparation of enzymatic reaction of the soy isoflavone aglycone β-glucosidase producing strains entirely feasible.Crude soy isoflavones by enzyme treatment,the number of aglycone increased significantly,particularly glycitein and genistein,respectively,an increase of 11.67 times and 30 times.To lay the foundation for further study.