Effect of specific connected ribozyme-mediated cleave on anti-HBV in vitro

AIM: To detect the cleavage activity of single andconnected ribozymes. METHODS: Three single ribozymesand a specific connected ribozyme (Rz 123 ) containing three ribozymes against hepatitis B virus core gene were designedwith computer, Then constructed transctiptional vector ofthree single ribozymes and transctiptional vector (pGEMRz123 ) containing connected ribozymes (Rz123 ) and 5'. 3'self-splicing ribozymes. Observed the cleavage activity ofthree single and connected ribozymes on target RNA. RESULTS: Three single antiviral ribozymes can cleave its targetRNA efficiently,and connected ribozyme was released properly from the transcript of pGEMRz123, following cleavage by5' and 3 cis-ribozymes. The result also demonstrated thatthree ribozymes of , connected ribozyme can not only cleave itstarget site respectively, but also worked cooperatively. theconnected ribozyme increased cleavage efficiency than the single ribozyme. CONCLUSION: Three single and connected ribozymes with computer design can cleave RNA of hepatitis Bvirus core gene. The conected ribozyme can cleave targetRNA combingly.