Under the reaction condition that/=2.4,polymerized hydroxyl Al ion prepared by Al3+ and alkali liquor and thereby inorgano pillared bentonite,organo pillared bentonite and inorgano-organo pillared bentonite prepared by acetyl trim ethylamine bromide(CTMAB).XRD result indicates that the interlayer spacing of bentonite is increased obviously and reaches to 1.9 nm or more.IR also indicated that Na-bentonite after polarization,pillaring species enter into the interlayer of bentonites.After studying the removing effect of inorgano-pillared bentonite,organo-pillared bentonite and inorgano-organo-pillared bentonite on the above four kinds of organism,the experimental result showed that the adsorption behavior of 3 kinds of pillared bentonites on organism is much better than raw bentonite.When dropping 0.2g,86.77%、97.26%、90.81% of nitrophenol was adsorbed.