灰葡萄孢菌
几丁质酶
茉莉酸甲酯
乙烯
葡萄球菌炎
发病相关蛋白
植物抗病性
生物
过氧化物酶
苯丙氨酸解氨酶
植物对草食的防御
茉莉酸
园艺
微生物学
植物
茄科
酶
生物化学
基因
拟南芥
催化作用
突变体
作者
Wenqing Yu,Ruirui Zhao,Jiping Sheng,Lin Shen
标识
DOI:10.1021/acs.jafc.8b03971
摘要
Methyl jasmonate (MeJA) and ethylene play important roles in mediating defense responses against Botrytis cinerea. Ethylene response factors (ERFs) are the final components of ethylene signal transduction; whether SlERF2 participates in disease resistance against Botrytis cinerea is unclear. The objective of this study was to investigate the role of SlERF2 in MeJA-mediated defense response by using both sense and antisense SlERF2 tomato fruit. Our results showed that both MeJA treatment and pathogen infection upregulated SlERF2 expression level. Overexpression of SlERF2 enhanced tomato fruit resistance against Botrytis cinerea. MeJA treatment increased ethylene production, promoted the activities of chitinase, β-1,3-glucanase, phenylalanine ammonia-lyase, and peroxidase, and elevated pathogenesis-related protein content and total phenolic content. Moreover, the effects of MeJA on disease response were reinforced in sense SlERF2 tomato fruit, while they were weakened in antisense SlERF2 tomato fruit. These results indicated that SlERF2 was involved in MeJA-mediated disease resistance against Botrytis cinerea in tomato fruit.
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