Copper-Modified Ti6Al4 V Suppresses Inflammatory Response and Osteoclastogenesis while Enhancing Extracellular Matrix Formation for Osteoporotic Bone Regeneration

Copper has been reported to promote bone regeneration by increasing osteogenesis and decreasing inflammation and osteoclastogenesis. However, information on the effects of copper on osteoporotic cells involved in bone regeneration is scarce in the literature. In the current study, Ti6Al4 V-6 wt %Cu (Ti6Al4 V–Cu) was fabricated by selective laser melting (SLM) technology, and the effects of copper on the behaviors of osteoporotic and nonosteoporotic macrophages, osteoclasts, and osteoblasts were evaluated by comparison with Ti6Al4 V. Our results showed that Ti6Al4 V–Cu inhibited the activation, viability, and pro-inflammatory cytokine secretion of osteoporotic macrophages and decreased osteoclast formation and down-regulated osteoclast differentiation-related genes and proteins of osteoporotic osteoclasts. Furthermore, the bone extracellular matrix formation of osteoporotic osteoblasts was up-regulated by Ti6Al4 V–Cu. In conclusion, SLM-fabricated Ti6Al4 V–Cu exhibited excellent anti-inflammation and antiosteoclast capability, optimized extracellular matrix formation, and holds great potential for bone regeneration in osteoporotic patients.