细胞破裂
化学
萃取(化学)
超声
均质化(气候)
色谱法
颜料
类胡萝卜素
水萃取
离心机
食品科学
有机化学
生物多样性
核物理学
物理
生物
生态学
作者
Nabil Grimi,Anthony Dubois,Luc Marchal,Sébastien Jubeau,Nikolaï Lebovka,Eugène Vorobiev
标识
DOI:10.1016/j.biortech.2013.12.011
摘要
This work studies the extraction of intracellular components from microalgae Nannochloropsis sp. with application of different cell disruption techniques, including pulsed electric field (PEF) (20kV/cm, 1-4ms, 13.3-53.1kJ/kg), high voltage electrical discharge (HVED) (40kV/cm, 1-4ms, 13.3-53.1kJ/kg), ultrasonication (USN) (200W, 1-8min, 12-96kJ/kg), and high pressure homogenization (HPH) (150MPa, 1-10 passes, 150-1500kJ/kg). The data evidence that electrically based disruption techniques (PEF and HVED) allowed selective extraction of water soluble ionic components and microelements, small molecular weight organic compounds and water soluble proteins. Microscopic and sedimentation stability analyses have shown that microalgae cells in HVED-treated suspension were noticeably agglomerated and could be easily settled in centrifuge. The electrically based disruption techniques were ineffective for delivery of pigments (e.g., chlorophylls or carotenoids) and their extraction required subsequent application of more potent disruption techniques. The obtained data have shown that HPH disruption technique was the most effective; however, this mode required the highest power consumption.
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