Rugged LC-MS/MS Survey Analysis for Acrylamide in Foods

The described liquid chromatography−tandem mass spectrometry (LC-MS/MS) method for the detection of acrylamide in food entails aqueous room temperature extraction, SPE cleanup, and analysis by LC-MS/MS. The method is applicable to a wide variety of foods. [13C3]acrylamide is the internal standard. The limit of quantitation is 10 ppb (μg/kg). Data were obtained in duplicate from >450 products representing >35 different food types. The variability in analyte levels in certain food types suggests that it may be possible to reduce acrylamide levels in those foods. Keywords: Acrylamide; exploratory; FDA; food survey; liquid chromatography−tandem mass spectrometry; LC-MS/MS; method