微气泡
体内
过氧化氢
过氧化氢酶
超声波
材料科学
生物医学工程
检出限
生物物理学
纳米技术
化学
酶
生物化学
色谱法
放射科
医学
生物
生物技术
作者
Emilia S. Olson,Jahir Orozco,Zhe Wu,Christopher Malone,Boem-Ha Yi,Wei Gao,Mohammad Eghtedari,Joseph Wang,Robert F. Mattrey
出处
期刊:Biomaterials
[Elsevier]
日期:2013-11-01
卷期号:34 (35): 8918-8924
被引量:91
标识
DOI:10.1016/j.biomaterials.2013.06.055
摘要
We present a new class of ultrasound molecular imaging agents that extend upon the design of micromotors that are designed to move through fluids by catalyzing hydrogen peroxide (H₂O₂) and propelling forward by escaping oxygen microbubbles. Micromotor converters require 62 mm of H₂O₂ to move - 1000-fold higher than is expected in vivo. Here, we aim to prove that ultrasound can detect the expelled microbubbles, to determine the minimum H₂O₂ concentration needed for microbubble detection, explore alternate designs to detect the H₂O₂ produced by activated neutrophils and perform preliminary in vivo testing. Oxygen microbubbles were detected by ultrasound at 2.5 mm H₂O₂. Best results were achieved with a 400-500 nm spherical design with alternating surface coatings of catalase and PSS over a silica core. The lowest detection limit of 10-100 μm was achieved when assays were done in plasma. Using this design, we detected the H₂O₂ produced by freshly isolated PMA-activated neutrophils allowing their distinction from naïve neutrophils. Finally, we were also able to show that direct injection of these nanospheres into an abscess in vivo enhanced ultrasound signal only when they contained catalase, and only when injected into an abscess, likely because of the elevated levels of H₂O₂ produced by inflammatory mediators.
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