化学
荧光
卡西亚
生命科学中的荧光
色谱法
荧光光谱法
量子力学
医学
物理
病理
中医药
替代医学
作者
Takanori Sugimoto,Yoko Wada,Shosuke Yamamura,Minoru Ueda
出处
期刊:Tetrahedron
[Elsevier]
日期:2001-12-01
卷期号:57 (49): 9817-9825
被引量:21
标识
DOI:10.1016/s0040-4020(01)00999-1
摘要
Abstract We synthesized fluorescence-labeled probe compounds bearing 6-((7-amino-4-methylcoumarin-3-acetyl)amino)-hexanoyl (AMCA, 1), 6-N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)-aminohexanoyl (NBD, 2), and 6-(4-((5-dimethylaminonaphthalene-1-sulfonyl)-amino))-hexanoyl (dansyl, 3) groups as the fluorescent functionality. In these probe compounds, NBD-type probe, 2, showed leaf-opening activity at 5×10−6 M. The bioactivity of 2 is one-fifth as strong as that of the natural product, potassium isolespedezate (6). We carried out the binding experiment using 1 in a plant body. Then, it was suggested that fluorescence-labeled probe compound directly bound to a motor cell in pulvina of Cassia mimosoides. And this binding was specific to C. mimosoides. Probe compounds cannot bind plant sections of other nyctinastic plants.
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