方解石
降水
碳酸酐酶
化学
碳酸钙
钙
胞外聚合物
生物矿化
碳酸盐
细胞外
无机化学
Crystal(编程语言)
核化学
化学工程
矿物学
酶
生物化学
细菌
地质学
有机化学
古生物学
物理
程序设计语言
生物膜
气象学
计算机科学
工程类
作者
Wei Li,Weishan Chen,Pengpeng Zhou,Lei Cao,Liang Yu
标识
DOI:10.1016/j.colsurfb.2012.08.042
摘要
The dynamics of calcium carbonate (CaCO3) precipitation induced by microbial intracellular or extracellular carbonic anhydrase (CA) at initial pH 6.0, 6.5, 7.0 and 8.0 were investigated through the gaseous diffusion method. The results indicated that both the intracellular and extracellular CA could promote CaCO3 precipitation. The Ca2+ ions in the enzymatic systems at initial pH 8.0 were completely deposited at 48 h, which were respectively 21 h, 15 h and 14 h earlier compared with that at initial pH 6.0, pH 6.5 and pH 7.0, indicating that higher pH favored CaCO3 precipitation in the experimental pH range, and was beneficial to the catalytic action of microbial CA on CaCO3 precipitation. In addition, XRD analysis indicated that the CaCO3 precipitates were mainly calcite crystals in the presence of microbial CA. With increasing deposition time, the crystals gradually changed from prism shape to pyramid-like or irregular polyhedral shape based on FESEM analysis.
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