生物
柑橘溃疡病
多胺氧化酶
柑橘×冬青
多胺
异位表达
橙色(颜色)
转基因
亚精胺
转化(遗传学)
柠檬黄单胞菌
过敏反应
菌丝体
微生物学
分子生物学
基因
植物
植物抗病性
生物化学
病菌
酶
园艺
细菌
遗传学
作者
Xing-Zheng Fu,Chuanwu Chen,Yin Wang,Jihong Liu,Takaya Moriguchi
标识
DOI:10.1186/1471-2229-11-55
摘要
Abstract Background Enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene. Results In the present work, an apple spermidine synthase gene ( MdSPDS1 ) was introduced into sweet orange ( Citrus sinensis Osbeck 'Anliucheng') via Agrobacterium -mediated transformation of embryogenic calluses. Two transgenic lines (TG4 and TG9) varied in the transgene expression and cellular endogenous polyamine contents. Pinprick inoculation demonstrated that the transgenic lines were less susceptible to Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus canker, than the wild type plants (WT). In addition, our data showed that upon Xac attack TG9 had significantly higher free spermine (Spm) and polyamine oxidase (PAO) activity when compared with the WT, concurrent with an apparent hypersensitive response and the accumulation of more H 2 O 2 . Pretreatment of TG9 leaves with guazatine acetate, an inhibitor of PAO, repressed PAO activity and reduced H 2 O 2 accumulation, leading to more conspicuous disease symptoms than the controls when both were challenged with Xac. Moreover, mRNA levels of most of the defense-related genes involved in synthesis of pathogenesis-related protein and jasmonic acid were upregulated in TG9 than in the WT regardless of Xac infection. Conclusion Our results demonstrated that overexpression of the MdSPDS1 gene prominently lowered the sensitivity of the transgenic plants to canker. This may be, at least partially, correlated with the generation of more H 2 O 2 due to increased production of polyamines and enhanced PAO-mediated catabolism, triggering hypersensitive response or activation of defense-related genes.
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