反转运蛋白
反转运蛋白
转基因作物
钾
液泡
钠氢反转运蛋白
钠
化学
转基因
拟南芥
植物
生物
园艺
分子生物学
生物化学
基因
膜
细胞质
突变体
有机化学
作者
Lei Yang,H. Liu,Shaomin Fu,Hui‐Ming Ge,Raphael Tang,Yanli Yang,H. H. Wang,H. X. Zhang
出处
期刊:Biologia Plantarum
[Institute of Experimental Botany]
日期:2017-12-01
卷期号:61 (4): 641-650
被引量:18
标识
DOI:10.1007/s10535-017-0724-9
摘要
The tonoplast and plasma membrane localized sodium (potassium)/proton antiporters have been shown to play an important role in plant resistance to salt stress. In this study, AtNHX1 and AtNHX3, two tonoplast Na+(K+)/H+ antiporter encoding genes from Arabidopsis thaliana, were expressed in poplar to investigate their biological functions in the resistance to abiotic stresses in woody plants. Transgenic poplar plants expressing either gene exhibited increased resistance to both salt and water-deficit stresses. Compared to the wild type (WT) plants, transgenic plants accumulated more sodium and potassium ions in the presence of 100 mM NaCl and showed reduced electrolyte leakage in the leaves under water stress. Furthermore, the proton-translocating and cation-dependent H+ (Na+/H+ or K+/H+) exchange activities in the tonoplast vesicles isolated from the leaves of transgenic plants were higher than in those isolated from WT plants. Therefore, constitutive expression of either AtNHX1 or AtNHX3 genetically modified the salt and water stress tolerance of transgenic poplar plants, providing a potential tool for engineering tree species with enhanced resistance to multiple abitotic stresses.
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