Engineered bacteria detect tumor DNA

细菌 生物 清脆的 水平基因转移 基因组 DNA 基因 结直肠癌 滚动圆复制 计算生物学 微生物学 癌症 DNA复制 遗传学
作者
Robert Cooper,Josephine A. Wright,Joseph Kim Fai Ng,Jarrad M. Goyne,Nobumi Suzuki,Young Kyung Lee,Mari Ichinose,Georgette Radford,E Thomas,Laura Vrbanac,Rob Knight,Susan L. Woods,Daniel L. Worthley,Jeff Hasty
标识
DOI:10.1101/2021.09.10.459858
摘要

Summary Advances in bacterial engineering have catalysed the development of living cell diagnostics and therapeutics 1–3 , including microbes that respond to gut inflammation 4 , intestinal bleeding 5 , pathogens 6 and hypoxic tumors 7 . Bacteria can access the entire gastrointestinal tract 8 to produce outputs measured in stool 4 or urine 7 . Cellular memory, such as bistable switches 4,9,10 or genomic rearrangements 11 , allows bacteria to store information over time. However, living biosensors have not yet been engineered to detect specific DNA sequences or mutations from outside the cell. Here, we engineer naturally competent Acinetobacter baylyi to detect donor DNA from the genomes of colorectal cancer (CRC) cells, organoids and tumors. We characterize the functionality of the biosensors in vitro with co-culture assays and then validate in vivo with sensor bacteria delivered to mice harboring colorectal tumors. We observe horizontal gene transfer from the tumor to the sensor bacteria in our mouse model of CRC. The sensor bacteria achieved 100% discrimination between mice with and without CRC. This Cellular Assay of Targeted, CRISPR-discriminated Horizontal gene transfer (CATCH), establishes a framework for biosensing of mutations or organisms within environments that are difficult to sample, among many other potential applications. Furthermore, the platform could be readily expanded to include production and delivery of antibiotic or antineoplastic therapeutic payloads at the detection site.
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