细胞周期蛋白依赖激酶
RNA聚合酶Ⅱ
细胞生物学
抄写(语言学)
细胞周期蛋白
发起人
细胞周期
细胞周期蛋白依赖激酶1
真核转录
生物
分子生物学
生物化学
细胞
基因表达
基因
哲学
语言学
作者
Mardo Kõivomägi,Matthew P. Swaffer,Jonathan J. Turner,Georgi K. Marinov,Jan M. Skotheim
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2021-10-15
卷期号:374 (6565): 347-351
被引量:32
标识
DOI:10.1126/science.aba5186
摘要
Cell division is thought to be initiated by cyclin-dependent kinases (Cdks) inactivating key transcriptional inhibitors. In budding yeast, the G1 cyclin Cln3-Cdk1 complex is thought to directly phosphorylate the Whi5 protein, thereby releasing the transcription factor SBF and committing cells to division. We report that Whi5 is a poor substrate of Cln3-Cdk1, which instead phosphorylates the RNA polymerase II subunit Rpb1’s C-terminal domain on S5 of its heptapeptide repeats. Cln3-Cdk1 binds SBF-regulated promoters and Cln3’s function can be performed by the canonical S5 kinase Ccl1-Kin28 when synthetically recruited to SBF. Thus, we propose that Cln3-Cdk1 triggers cell division by phosphorylating Rpb1 at SBF-regulated promoters to promote transcription. Our findings blur the distinction between cell cycle and transcriptional Cdks to highlight the ancient relationship between these two processes.
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