CD44细胞
微泡
外体
癌症研究
化学
材料科学
表面等离子共振
流式细胞术
生物物理学
纳米技术
纳米颗粒
细胞
分子生物学
医学
生物
生物化学
小RNA
基因
作者
Abhimanyu Thakur,Chunping Xu,Wing Kar Li,Guangyu Qiu,Bing He,Siu Pang Ng,Chi‐Man Lawrence Wu,Youngjin Lee
标识
DOI:10.1016/j.bios.2021.113476
摘要
Glioblastoma (GBM) is the fatal brain tumor in which secreted lactate enhances the expression of cluster of differentiation 44 (CD44) and the release of exosomes, cell-derived nanovesicles (30–200 nm), and therefore promotes tumor malignant progression. This study found that lactate-driven upregulated CD44 in malignant Glioblastoma cells (GMs) enhanced the release of CD44-enriched exosomes which increased GMs' migration and endothelial cells’ tube formation, and CD44 in the secreted exosomes was sensitively detected by “capture and sensing” Titanium Nitride (TiN) - Nanoholes (NH) - discs immunocapture (TIC) - atomic force microscopy (AFM) and ultrasensitive TiN–NH-localized surface plasmon resonance (LSPR) biosensors. The limit of detection for exosomal CD44 with TIC-AFM- and TiN–NH-LSPR-biosensors was 5.29 × 10-1 μg/ml and 3.46 × 10-3 μg/ml in exosome concentration, respectively. Importantly, this work first found that label-free sensitive TiN–NH-LSPR biosensor could detect and quantify enhanced CD44 and CD133 levels in immunocaptured GMs-derived exosomes in the blood and the cerebrospinal fluid of a mouse model of GBM, supporting its potential application in a minimally invasive molecular diagnostic for GBM progression as liquid biopsy.
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