Objective To obtain the basic data of physiological and biochemical characteristics of Beagle dog's blood which are in different sex and age,and the weight of main organs and their coefficient of 8~12 months beagle dogs were measured,for pharmaceutical and toxicological experimental references.Methods Blood samples were taken from over 3000 Beagle dogs of different age and sex.Physiological and biochemical characteristics were examined by HITACHI-7020 automatic chemistry analyzer and SWELAB-AC920EO automatic hematology analyzer.The weight of main organs and their coefficient of 128 beagle dogs were measured.Results and Conclusion Basic data of physiological and biochemical characteristics of different sex and age,and the weight of main organs and their coefficient of Beagle dog's were obtained.